LOCUS       pLV-[hsa-miR-1289-2]        9165 bp    DNA     circular     27-JUN-2007
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7086..7946
                     /label=Amp(R)
     promoter        8147..8687
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5691..5925
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6500..6955)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    5997..6127
                     /label=SV40 pA
     misc_feature    6128..6341
                     /label=SV40 ori
     misc_feature    5012..5608
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..3630
                     /label=intron
     CDS             3677..5002
                     /label=rPuro (RFP Puro)
     misc_RNA        3110..3420
                     /label=hsa-mir-1289-2 precursor
BASE COUNT     2174 a      2404 c      2473 g      2114 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgaggatcca gaaggaacca 
     3121 aagccttgtc tgtcctgatg gcagaagttg caaccctgta gggcttctca acatgtgatc 
     3181 taagaccaca gctttgtaac ctaactcagc cacggtccta gttaaaaagg cacattccta 
     3241 gaccctgcct cagaactact gaacagagtc actgggtgtg gagtccagga atctgcattt 
     3301 ttacccctat cgcccccgcc attcccgccc ttccccccgc cattgcgtgc actaattgct 
     3361 ggaagcactc atgaggagag ccgcctgtgt tgtgtagaac tcccaccccc gcaacacaca 
     3421 gctagctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg 
     3481 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa 
     3541 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca 
     3601 gtggttcaaa gtttttttct tccatttcag gtgtcgtgaa aactacccct ctagagtcga 
     3661 gctaccggtc gccaccatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga 
     3721 gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga 
     3781 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa 
     3841 gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa 
     3901 ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg 
     3961 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga 
     4021 ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc 
     4081 ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat 
     4141 gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg 
     4201 cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc 
     4261 cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat 
     4321 cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta 
     4381 caaggaccca ccggtcgcca ccatgaccga gtacaagccc acggtgcgcc tcgccacccg 
     4441 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac 
     4501 gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt 
     4561 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt 
     4621 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc 
     4681 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct 
     4741 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga 
     4801 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg 
     4861 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg 
     4921 gctcggcttc accgtcaccg ccgacgtcga gtgcccgaag gaccgcgcga cctggtgcat 
     4981 gacccgcaag cccggtgcct gagcggccgc gtcgacaatc aacctctgga ttacaaaatt 
     5041 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct 
     5101 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg 
     5161 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc 
     5221 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt 
     5281 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc 
     5341 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg 
     5401 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg 
     5461 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc 
     5521 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg 
     5581 atctcccttt gggccgcctc cccgcctgga attcgagctc ggtaccttta agaccaatga 
     5641 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc 
     5701 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag 
     5761 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat 
     5821 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact 
     5881 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc 
     5941 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact 
     6001 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata 
     6061 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc 
     6121 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag 
     6181 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc 
     6241 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac 
     6301 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa 
     6361 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct 
     6421 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc 
     6481 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg 
     6541 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc 
     6601 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc 
     6661 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt 
     6721 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag 
     6781 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg 
     6841 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag 
     6901 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg 
     6961 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa 
     7021 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga 
     7081 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc 
     7141 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg 
     7201 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc 
     7261 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat 
     7321 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg 
     7381 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag 
     7441 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa 
     7501 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc 
     7561 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca 
     7621 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc 
     7681 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc 
     7741 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg 
     7801 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta 
     7861 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag 
     7921 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga 
     7981 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc 
     8041 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 
     8101 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 
     8161 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 
     8221 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt 
     8281 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 
     8341 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 
     8401 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 
     8461 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 
     8521 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 
     8581 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 
     8641 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 
     8701 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 
     8761 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 
     8821 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 
     8881 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca 
     8941 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga 
     9001 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt 
     9061 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca 
     9121 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgc 
//
